Tricine–SDS-PAGE is commonly used to separate proteins in the mass range 1–100 kDa. It is the preferred electrophoreticsystem for the resolution of proteins smaller than 30 kDa. The concentrations of acrylamide used in the gels are lower than inother electrophoretic systems. These lower concentrations facilitate electroblotting, which is particularly crucial for hydrophobicproteins. Tricine–SDS-PAGE is also used preferentially for doubled SDS-PAGE (dSDS-PAGE), a proteomic tool used to isolateextremely hydrophobic proteins for mass spectrometric identification, and it offers advantages for resolution of the seconddimension after blue-native PAGE (BN-PAGE) and clear-native PAGE (CN-PAGE). Here I describe a protocol for Tricine–SDS-PAGE,which includes efficient methods for Coomassie blue or silver staining and electroblotting, thereby increasing the versatility ofthe approach. This protocol can be completed in 1–2 d.http://bbs.bbioo.com/attachment.php?aid=23377&k=ee7e6eb523c8d93e049e06adbc0420f1&t=1258167150&fid=139&sid=ca14IPPbj3Ee9ZCKH2i89cRhOw6KuW%2FmSRqWkHaemyuU2uw[此贴子已经被作者于2009-11-14 11:06编辑过]全球肽网客户